Transport of glutathione-conjugates in human erythrocytes.
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چکیده
منابع مشابه
Transport of glutathione-conjugates in human erythrocytes.
The last step of detoxification of both endogenous and environmental toxicants is typically a conjugation that produces a bulky hydrophilic molecule. The excretion of such conjugates out of cells is of sufficient biological importance to have led to the evolution of ATP-driven export pumps for this purpose. The substrate specificity of such transporters is broad, and in some cases it has been s...
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Human erythrocytes were incubated with 3H-glycine to label the glutathione pool. These cells were then used to determine the rate of oxidized glutathione (GSSG) transport out of erythrocytes. For 6 normal individuals, the mean transport rate was 6.7 nmole GSSG/hr/ml red cells. This transport rate would suggest a half-life of 4.7 days for the erythrocytic glutathione, which is in close agreemwnt...
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The reduced glutathione of normal and glucose-6-P dehydrogenase-deficient human erythrocytes was oxidized by subjecting the cells to HzOz diffusion or to treatment with methyl phenylazoformate. A portion of the oxidized glutathione thus formed disappeared from the erythrocytes and could be recovered from the medium when the normal cells were incubated in the absence of glucose or when glucose6-...
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Purified inside-out vesicles from human erythrocytes were used to investigate the active transport of oxidized glutathione (GSSG). Incubation of vesicles and GSSG in the presence of ATP resulted in the transport of GSSG into the vesicles. When vesicles were incubated with reduced glutathione (GSH), no transport was observed. At GSSG concentrations of less than 5 mM, transport was linear up to 4...
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The mechanism of transport of the antiviral agent acyclovir (ACV) into human erythrocytes has been investigated. Initial velocities of ACV influx were determined with an "inhibitor-stop" assay that used papaverine to inhibit ACV influx rapidly and completely. ACV influx was nonconcentrative and appeared to be rate-saturable with a Km of 260 +/- 20 microM (n = 8). However, two lines of evidence ...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 2000
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.2000_3993